A picture of her betting slip shows Sophie put small bets on horses Tomyris, Cracksman, Archetype and Headway on day two of the famous annual horse racing meet.
Tomyris could only finish fifth in the Duke of Cambridge Stakes at 3.40pm, while Cracksman suffered a shock defeat in the Prince of Wales Stakes at 4.20pm when he was pipped to the post by Poet’s World.
The Countess’ worst result of the day came when Headway could only finish a lowly 29th out of the 30 horses that crossed the line in the Royal Hunt Cup at 5pm, while in the Jersey Stakes 35 minutes later, Headway finished down in eighth.
Despite the disappointments, Sophie, who looked stunning in an aqua blue Emila Wickstead jumpsuit with matching hat and long-drop earrings, appeared to be enjoying herself, along with several other members of the Royal Family.
It was her second successive day at the famous racecourse having arrived for the start of Royal Ascot yesterday, with the annual horse racing event running through until Saturday.
This week has been notable for the debut of the Duchess of Sussex, Meghan Markle, at the races on Tuesday, her first visit to Royal Ascot as a member of the Royal Family.
Meghan and her new husband Prince Harry, the Duke of Sussex, travelled into Ascot Racecourse with the Earl and Countess of Wessex in a horse drawn carriage.
Also in attendance were the Queen, the Prince of Wales and Duchess of Cornwall, Princess Anne and Princesses Beatrice and Eugenie.
The Countess’ bet on the four horses came one day after she celebrated her 19th wedding anniversary to Prince Edward, the Earl of Wessex.
They married on June 19, 1999 at St George’s Chapel at Windsor Castle, which is where the Duke and Duchess of Sussex also enjoyed a glittering ceremony last month when they tied the knot in front of delighted onlookers.