Footage from the ceremony shows Meghan stick her hand out in her husband Harry’s direction as the pair walked behind the Queen during the ceremony at Buckingham Palace.
Harry then can be seen quickly clapping his hands together as if nothing had happened.
The pair in the lead up to the Royal Wedding had been know for the affectionate gestures in public which attracted criticism from body language experts.
Robin Kermode, who trains professionals around the world on body language, had previously said: “The Queen rarely holds hands with her husband in public and this seems to have set an unwritten precedent for the other royals.
“It is very clear that William and Kate are emotionally close, however, they do not seem to feel the need to prove their love — particularly when on official state business.”
However royal fans have defended the Duchess, with one Instagram user writing: “Leave the girl alone.”
Another commented: “She isn’t trying to hold his hand.”
“Why can’t they just hold hands?” questioned one person.
There is no royal rule saying royal couples cannot hold hands and during Meghan’s debut at Ascot last week the pair was inseparable.
Meghan was also accused of breaking royal protocol by social media users when she crossed her legs at Tuesday event with the Queen.
One royal fan wrote: “The Duchess of Sussex has her legs crossed wrong. What a disrespect to the Queen. All royal ladies cross at the ankles or put both legs to the side.”
Another person said: “Meghan don’t cross your legs Queen doesn’t like that.”
However, etiquette expert William Hanson has said there is nothing wrong with the way Meghan chose to sit and it is actually a position favoured by a number of royals.
The Queen herself has even been pictured crossing her legs at the knees during a BBC interview about her Coronation which aired in January.
Mr Hanson said: “There was nothing disrespectful about HRH The Duchess of Sussex’s seating position last night.
“She had chosen to use the traditional ‘finishing school’ technique of crossing one leg over the other, resting one ankle against the edge of the shin.
“This used to be used by ladies who wished to show off a grand ballgown and today it can be a good way to highlight a pair of hosiery-clad legs.
“Royal women do not often cross their legs, although HRH The Duchess of Cornwall sometimes does; this tip is to ensure nothing gets flashed that shouldn’t be!”